Lipid structures disappear upon negative stainging. Does anyone knows this effect?

13 December 2020 1,559 3 View

Cryo-EM Vitrobot settings for large lipid structures. How can I reduce ice thicknes?

Dear all, I have an issue figuring out good plunging conditions for large lipid structures: I have carbon coated C-Flat 2/2 400mesh copper grids with large (elongated) lipid structures on it....

13 December 2020 6,696 2 View

Can I use lentiviral packaging HEK293T cell lines to produce stable clones?

Hi, I generally made LV in HEK293T cell lines by triple transfection. I am wondering whether the newly generated LV will transduce the packaging HEK293T. In this way I could exploit the...

09 September 2020 6,333 3 View

Which software is the best for scientific presentations in which equations occupy a big part of it?

I usually use Beamer, in which equations are easy to write (and I already have them in Latex) but it's too static and rigid. In Powerpoint it is impossible to write the equations, it takes a lot...

15 July 2020 8,260 3 View

How to calculate the max Jsc for a given Eg using spectral irradiance [W/(m2*eV)] data?

I'm calculating the max available Jsc as a function of bandag Eg. For that I'm using the following integral: Jsc=q*int(f(Eph)*EQE.dEph) from Eg to \inf With: q=1C/eV f(Eph) Spectral Irradiance...

07 July 2020 5,873 4 View

Inverted current Mirror connection in LM741?

Analyzing the topology of the LM741 , the current mirror (Q8-Q9) that bias the first stage of the op. amp. is not connected as usual (see the red circle on the picture). Curiosly, this way to...

24 January 2020 8,419 2 View

Negative values in anti-inflammatory assay: inhibition of albumin denaturation?

I am performing in vitro anti-inflammatory assay: inhibiton of albumin denaturation by organic extract and purified fractions from natural material. Since my samples are colored (red, orange,...

14 September 2019 2,528 1 View

Can you help me with Python script?

Hi to all, I have an approaching deadline and to perform my analysis (design guide RNAs) I need to insert a lot of mutations in my sequenze (txt file). Mutations are inside a excel in file...

05 February 2019 2,494 1 View

What are good color palettes for students in the autism spectrum?

I've read that, for students in the spectrum, it may help to design a class environment that avoids overstimulating their cognitive system with unnecessary distractions, one of them begin colorful...

28 January 2019 2,557 3 View

How to improve undergraduate and postgraduate educational settings with gamification?

Gamification is a brilliant strategy to support the development of educational settings, especially in undergraduate and postgraduate courses. Therefore, I would like to ask you what do you think...

22 October 2018 2,456 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

How to accurately measure cytokines levels for my university research proposal?

Hello, i am writing a theoretical research proposal for one of my university assignments regarding Covid-19. The brief is pretty relaxed and i have chosen to conduct a study on how the...

11 February 2021 908 5 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View

Calculating the CFU?

i am constructing research for the antibacterial activity of the medication and I am testing it by %RCC, so my Q after I did 10 serial dilutions for the bacterial suspension can we do plating for...

04 February 2021 5,886 1 View

What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View

What is the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow endothelial cells?

Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...

28 January 2021 8,765 4 View

What M1 and M2 markers can I use for flow cytometry ?

Hi, I'm a student and I'm doing an internship. I need to asses the M1 and M2 populations of a culture using flow cytometry. From my researches, I know that this is subject to some controversy, as...

26 January 2021 299 3 View

Does re-stimulation of T cells with PMA and Ionomycine before staining for intracellular cytokines for flow cytometry affect reliability of the assay?

I understand that to be able to measure intracellular cytokines there is a need to restimulate with PMA and Ionomycin but I don't understand how the results will be reliable after that. PMA and...

18 January 2021 7,240 7 View