Could you kindly provide insights into suitable techniques or protocols for achieving effective protein fraction drying following acetone precipitation, considering the limitations associated with freeze drying? Your expertise and recommendations in this matter would be immensely valuable to the progress of my research.
I usually directly precipitate the protein with TCA + acetone wash. I'm not sure if this is what you have been doing.
But the protein pellet after 2-3 acetone washes should remain at the bottom of a tube as a pellet. And I just let it air dry (open the tube cap and just let sit at a bench or in a ventilated hood). No lyophilization needed. Note the protein will be denatured.
Alternatively, methanol-chloroform precipitation is also frequently used for protein precipitation.
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