How can I add multi-label for an image after region segmentation ?

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Can we add midori green directly while preparing polyacrylamide gels?

Can we add midori green directly while preparing polyacrylamide gels? or should only be done as a post staining after running the PAGE gel?

08 April 2024 2,203 3 View

Poor migration of PCR products in a 3.2 % agarose gel?

Hello all, I have a query in resolving close products in agarose gel electrophoresis: I have three different expected products in my samples: 460bp, 480bp (only in mutation), 323bp, and I run them...

02 April 2024 1,141 3 View

Ferrous fumarate release from sustained release formulation with Topioca starch as a release limiting ingredient?

Hi Team, While formulating the sustained formulation for the active ferrous fumarate with the Tapioca starch as rate/release limit ingredient in the process. Process: Dry mixing , coated tablet,...

09 May 2023 1,488 0 View

Roche Lightcycler 480 error?

I get an error during relative quantification analysis in Lightcycler 480 software: Error calculating subordinate analysis, index exceeds matrix dimensions. Has anyone encountered and solved this...

10 December 2022 5,983 0 View

Reprogramming fibroblasts with episomal vectors_how often is Vector integration possible?

I am reprogramming human dermal fibroblasts to iPSCs using Yamanaka factors with Nucleofection method. All obtained iPSC clones shows integration of one or a few plasmids after passage 6 of the...

14 August 2021 1,158 3 View

Is there a good online tool available to check the strength of Kozak consensus sequence for translation initiaiton, given the amino acid sequence?

I have an amino acid sequence of my target (KO) and there are different potential ORFs that could be initiated, as predicted by expasy-translate. Is there a way to check the strength of the Kozak...

03 May 2021 7,347 5 View

How to detach iPSC colonies from reprogrammed fibroblasts?

I am reprogramming fibroblasts to iPSCs. I see small iPSC colonies around D21 and want to isolate them. I have two questions here. 1. Many iPSC colonies are already yellow from the center. Does...

01 March 2021 9,917 5 View

Can any one help me in analyzing the quarter car model developed using simscape toolbox in MATLAB?

Model has been developed using Simscape. I want to analyze the system outputs and Animate the same.

03 February 2016 4,939 1 View

Is there any technique for genetic modifications or biochemical modifications of metschnikowia yeasts for enhancing lipid accumulation?

I have Metschnikowia pulcherrima strain growing in distillery spent wash and collecting lipids for bio-diesel production. Currently the yield is a little low, despite the high sugar contents and...

13 January 2016 1,835 2 View

How can I calculate the energy density and power density ?

I have calculate the specific capacitance for supercapacitor, i have used three electrode cell system, i need energy density and power density formula? Can you give some ideas..

15 July 2015 6,539 16 View

Stability of the Solar System: Insights from Einstein’s Equations ?

The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...

07 August 2024 2,569 1 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

Why does everyone use vs code?

Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...

07 August 2024 7,013 4 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

How to Compress Information Neurally?

Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...

01 August 2024 4,456 2 View

What exactly is RAG-LLM doing? Isn’t it data engineering?

What exactly is Retrieval Augmented Generation for Large Language Model doing? Isn’t it data engineering?

30 July 2024 7,376 3 View

May I know the exact Quartile of the journal- Advanced Engineering Materials (Wiley) for material science category?

In some data sources it has been grouped in Q1 and some shows it is Q2.

29 July 2024 4,227 2 View

I need the housing or real estate prices data since 1950 till date in India. Can anyone guide how can I get this data ?

I didn't find any data source for continuous time series since 1950. NHB Residex and RBI give data from 2013 and 2010 onwards. Please guide. Thanks !!!

27 July 2024 6,271 5 View