How can I account for gene copy number in qpcr biomass measurements (fungal community)?

Fabiola Pulido-Chavez @Fabiola-Pulido-Chavez

13 June 2021 1 1K Report

Hi everyone,

I performed qPCR analysis using the FungiQuant primers to target the 18S region for fungi to determine fungal biomass. I used a plasmid standard containing this region from S. cerevisiae and was able to get some biomass measurements. However, I am unsure how to account for variation in gene copy numbers. If anyone has any ideas or can guide me to an article, I would greatly appreciate it.

Abhijeet Singh

Simple answer: you can't.
Complex answer: it requires details of all fungal species present in your sample and information about each genome and gene copy number, followed by some calculations to correct for copy numbers. Unless you have all these things, you can't correct for copy number variation in your analysis.

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