How can get rid of primer dimer?

More Maedeh Shabani's questions See All

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Does anybody know how atlocal command in Comsol works?

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Frederic Lepretre

Hi Maedeh Shabani

more informations on what you're planing to do could help answering...

from what I see, on genomic you'll have more than 16kb to amplify, on mRNA for qRT-PCR you'll get 182Bp, a little bit larger (around 100 is a good point). changing positions of your primers should thereby be better, since I can't see on your primers sequence what could be wrong. are you sure that your target is expressed in your samples? on GTEX portal your target seems silent in some tissues, maybe the problem...

see attachments for help.

all the best

fred

maybe an other issue on exon expression in your samples, since exon 1 and 2 of your target seem absent in some tissues. see attachment.

Maedeh Shabani

thanks a lot Frederic Lepretre for answering my question, actually I work on placenta tissue and whole blood and I checked the expression in NCBI but I didn't check on GTEX.