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Hello, I am working on lncRNAs, one of my lncRNAs is INHBA-AS1 and I design intron spanning primer for it. In real time PCR I just have primer dimer with melt lower than 70, I decreased primers...
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I want to design primer for lncRNA but lncRNAs have alternative splicing and because of that I don't know how I design primer for them?
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Hello, I am going to work on lncRNA biomarkers in preeclampsia, I wanted to know how stable lncRNAs are in the blood? And how many hours after sampling can I extract lncRNAs from the whole blood?...
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Hello, I want to do sanger sequencing for 3 SNPs in the CETP gene. Two of them are located in exons and one of them in intron. What should I consider for designing primers?
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