4 Questions 2 Answers 0 Followers
Questions related from Maedeh Shabani
Hello, I am working on lncRNAs, one of my lncRNAs is INHBA-AS1 and I design intron spanning primer for it. In real time PCR I just have primer dimer with melt lower than 70, I decreased primers...
09 November 2022 4,082 3 View
I want to design primer for lncRNA but lncRNAs have alternative splicing and because of that I don't know how I design primer for them?
13 April 2022 9,385 2 View
Hello, I am going to work on lncRNA biomarkers in preeclampsia, I wanted to know how stable lncRNAs are in the blood? And how many hours after sampling can I extract lncRNAs from the whole blood?...
10 March 2022 8,488 2 View
Hello, I want to do sanger sequencing for 3 SNPs in the CETP gene. Two of them are located in exons and one of them in intron. What should I consider for designing primers?
20 December 2021 3,119 2 View
