10 October 2017 1 133 Report

(How) can DMT and Play promote emotional regulation with adolescents (12-17 years old) with ADHD?

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How to improve 260/230 ratio in rna extraction for PCR?

The 260/280 ratio is ok between 1.8 and 2.0 however we were unable to advance due to the 260/230 ratio

13 May 2024 7,643 3 View

The Structure Harvester website still not working?

I'm trying to run the Structure Harvester for interpretation based on Evanno method and the website don't work. Someone have any alternatives? Thank you

12 April 2024 2,757 1 View

Why the best blocking solution to blot?

We already used milk: 5% to 1 hour, 7% to 2 hour, 10% to 1h, 5% overnight, but, , in all blockages it still marks the entire membrane, every run, there is a large band with no specific mark HELP

20 February 2024 7,346 0 View

What is the best way to homogenize spinal cord for blot?

I'm going to use the RIPA buffer, but I don't know if I should use the sonicator or polytron homogenizer

30 January 2024 7,432 1 View

Error in autodock 4 + pyrx?

"ERROR! Too many atom types have been found: maximum is 14; we cannot continue" Does anyone know how to solve it?

27 January 2024 6,988 5 View

What is the best way to do a cytokine blot?

I am trying to do a western blot of cytokines (iL-10, IL-6) of muscle tissue, using a strip of lysis buffer, 50 ug of proteins and blocking in 5% skimmed milk, however no signal appears, any tips?

24 January 2024 7,021 2 View

What is the best software to analyze genetic diversity for large SNP dataset (~67K SNPs)?

I need to calculate number of different and effective allele for a diversity study using large SNP dataset. Normally I use GenAlex but I can't load the data due to limited no of columns. Please...

01 January 2024 6,128 2 View

Are there elucidated signaling pathways involved in the differentiation of memory T cell subsets?

We are aware that T cells can specialize into various memory cell types, such as stem cell memory (Tscm), central memory (Tcm), effector memory (Tem), and resident memory (Trm) subsets. However,...

22 November 2023 683 1 View

How can I confirm that my cell culture is pure?

How can I confirm that the cells isolated from the abdominal cavity of rats, following peritoneal lavage with dPBS and antibiotics, and subsequently centrifuged and plated, are indeed macrophages,...

22 November 2023 224 3 View

What is the best way to quantify astrocytes and microglia in tissue?

I need to quantify microglia and astrocytes through immunofluorescence, however I would like to know the best way to quantify, whether fluorescence intensity or manual counting or some other type

19 November 2023 212 3 View

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