To detect Pseudomonas in drinking water, collect samples carefully in sterile bottles and filter them through a fine 0.45 µm membrane. Grow the filtered sample on selective media like Cetrimide agar for 24–48 hours at body temperature. Colonies often appear blue-green or yellow-green and have a distinctive grape-like smell. Confirm their identity with simple tests such as oxidase positivity, Gram staining, and growth at 42 °C. For more certainty, use biochemical kits, MALDI-TOF, or PCR targeting specific genes like oprL. Count the bacteria as CFU per 100 mL, ensuring proper controls to guarantee reliable results.

Pseudomonas spp. in water samples can be diagnosed using membrane filtration followed by culture on selective agar, biochemical confirmation tests, and rapid molecular or immunoassay-based methods for fast and specific detection.