Can you share the procedure for the same.
The harmful Dye pollution from industrial processes such as textile generated how to remove with magnetic biochar ?
07 July 2024 8,118 1 View
I am planning to purify the mononucleosome for cryoEM study. I have seen, people assemble the nucleosome from recombinantly expressed histones and then providing DNA sequence to it. Isn't this...
19 May 2024 7,170 1 View
I have a recipe of loading dye (10 mM EDTA, 1X TBE, pinch of Xylene cynol and Orange G dye which is made upto 10 ml with 100% Formamide) but somehow the bands of the nucleotide product...
17 April 2024 8,025 6 View
Purification protocol for human PARP1 with good yield.
13 March 2024 9,746 3 View
I have prepared the FRAP reagent according to Benzie & Strain (1996). My samples are essential oil nanoemulsion. However, after adding the FRAP reagent into the sample, it doesn’t turn blue....
05 February 2024 2,765 1 View
Tilapia and Common carp can breed many times while Indian Major Carp breed mostly during monsoon, then is it due to Genetic factors or environmental reasons?
13 November 2023 9,424 2 View
I want to use two different datasets where one data will be for control while another data will for test samples. How can I use it. What will be the protocol including normalisation. Kindly help...
11 November 2023 4,388 1 View
Any specific technique, please suggest to me?
04 October 2023 8,796 4 View
Dear all, I have been trying to knockdown using siRNA (duplex) targeted against my protein of interest (# 2 lane shown in the image) and a random non-target siRNA ((# 1 lane shown in the image)...
29 September 2023 765 2 View
during nreaction after 1 h , we saw O2, N2 peaks in GC, our reaction is pyrolysis abive 500C. It is hard to figure out where these compounds are coming either from the reactor or the catalyst?
31 August 2023 6,323 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View
As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...
31 July 2024 9,859 0 View
