How can determine the DS for chitosan-g-folic acid using UV-Vis spectrophotometry?

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Does anyone know a source for theta-replicating Staphylococcus plasmids that are compatible?

I am trying to put 3 separate plasmids into Staphylococcus aureus and I am looking for those that are theta replicating and compatible. I have obtained pSK9065 (from pSK1) from Addgene, but the...

23 July 2024 6,968 0 View

How to maintain intestinal tissue resident cells (TRM) in culture?

I am attempting to culture tissue resident T cells (TRM) derived from fresh intestinal tissue for up to six days. I am finding that the viability of the T cells is low at D0, and a very small...

08 July 2024 4,887 2 View

Help with in vitro testing of candida biofilm susceptibility?

Hi all, Would anyone have any suggestions for articles and protocols for performing microdilution tests on candida biofilm? Do you recommend diluting the drug in the medium and adding it to the...

19 June 2024 4,420 3 View

How to reconstitute ATCC of B.ovatus?

I am trying to reconstitute the bacterium B.ovatus ATCC. it was first placed in a THIO broth and despite this we observed no turbidity. This was done 24h after the stick containing B.ovatus ATCC...

12 June 2024 1,644 3 View

Someone can help me with error prone PCR?

How can you regulate the number of random inserted mutations in error prone PCR?

14 May 2024 673 2 View

How do I mention translation in an IPA research project?

Good evening, My IPA (Interpretative Phenomenological Analysis) research project involves conducting interviews in a different country and in a different language. Considering that I will need to...

05 May 2024 6,147 2 View

False Discovery Rate -- how many p values do you correct?

I am using this code (below) to use FDR to correct p values because I ran three exploratory growth curve models. How many p values do you need to correct? Do I just correct all of the p values for...

02 May 2024 4,028 4 View

Help to detect aggrecan in WB, anyone has detected the 250KDa band in mouse brain homogenates?

We are interested in detecting aggrecan (250 KDa) by WB. No luck so far, are there any recommendations, such as running buffer, sample loading buffer? Thank you in advance!

05 April 2024 6,269 4 View

How design Specific Primers for Reverse Transcription?

Hi :) How should I design primers for (-)ssRNA samples from Influenza A and hRSV viruses? I need to select the protein, locate the gene sequence, and then use it to design primers. Should I...

04 April 2024 4,896 1 View

Can I use feces preserved in formaldehyde to perform the McMaster technique?

Can I use feces preserved in formaldehyde to perform the McMaster technique for Haemonchus research?

20 March 2024 1,339 0 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal?

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal? Kindly advice

04 August 2024 7,367 3 View

Are authors sowing for scientific Journals to be reaping the benefits? We are charged for publication but we offer free peer review services, why?

As authors and academic writers, we usually prepare our manuscript using our own resources. We submit these manuscripts to scientific Journals for peer review and publication sometimes at a fee....

03 August 2024 7,304 2 View