Hello,
I am trying to differentiate HEK293 cells to induce ciliogenesis. I have seen in papers that this is possible by serum starvation but I obtain a mix population of differentiated cells and many dividing cells.
Thank you very much in advance!
Usually this is done by adding a low concentration of serum (1 - 2% FBS) to the medium and then incubating for 48 hours. It is also possible to add chemicals that promote ciliogenesis.
https://www.researchgate.net/post/How-to-promote-ciliogenesis
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