How can antibodies detect SRY (sex-determining region Y) protein?

22 February 2017 1 6K Report

Subcellular location of SRY (sex-determining region Y) protein is in the nucleus and cytoplasm of Y-chromosome bearing sperm (http://www.uniprot.org/uniprot/Q03255). So how can antibodies act against SRY (sex-determining region Y) protein?

Adam B Shapiro

Cells are fixed and permeabilized for immunocytochemistry, so all parts of the cell are accessible to antibodies.

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