first you need to define meaning of localized translation, where do you want to see in a cell line which do not express this mRNA or in whole animal. Further you have to know where is it expressed in an organelles, cytoplasm and or nucleus.

1. I will do insitu hybridization can detect where the RNA is made in tissue sections.

2. To determine the protein expression I will do the following, isolate cytoplasm and nuclear extract to detect in these two compartments.

3. If it is cytoplasmic, I will use few cell organelle markers to immunolocalize with the the antibody against the protein you are interested.

4. I would also fractionate the protein using dicontinuous sucrose gradient to check the density sedimentation patterns and use organelle specific markers to identify.

5. Finally I would like to silence this RNA in a mammalian cell line and corroborate the above all experiments by repeating those experiments.

Best

Kumar