Can I use the following standards to quantify strigolactone in Bambara groundnut:
1. 5-Deoxystrigol
2. Orobanchol
3. Strigol
4. Orobanchyl acetate (Alectrol)
I have noticed two standards that seem to have two functions in one for example(±)2´-Epi-orobanchol can be used in synthetic preparation and synthesis of germination stimulants (±)-orobanchol and (±)-strigol via allylic rearrangement.. (±)2´-Epi-5-deoxy-strigol is said to ba s a derivative of Strigol which is a germination stimulant. It can be used in synthetic preparation of (±)-5-Deoxystrigol using reductive carbon-carbon bond formation. If I buy and use them as standards will they give me the results I need? I had also noted that the above standards also come with the +and - to their example (±)-orobanchol and (±)-strigol. Does (±)-5-Deoxystrigol joint word and (±)-5-deoxy-strigol function the same.
Which machine can I use to get the best results? HPLC or LC-MS, please advise me
Dear Rudo,
Use LC-MS, if you want to have chiral discrimination you need to use chiral column.
Best wishes,
Sami
They will provide the estimation for their structural equivalents and will need to be estimated separately to provide better results. For the isomer present, a chiral column will be best suited if you will be using the racemate, or the specific isomer, although you can also run a non-chiral or at least an RP column. It would be better to buy and estimate each isomeric product separately on a chiral column. The LC-MS is not of much use in this case, a HPLC should do the job.
- Badges
- Science method