I have few queries wrt HLA alleles
1. Apart from homologous recombination what are the molecular consequences which lead to difference in HLA alleles.
2. What are the major differences between HLA alleles.
3. How are HLA alleles different amoung MHC
4. What are the essential set of HLA to be screened before transplantation.
Please share your views and references
I hope, the Research Article by Stéphane Buhler & Alicia Sanchez-Mazas will provide answer to your questions.
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0014643
A good question for me is that this area is concerned with the transplantation of the organs and its course of effect and the implications of all segments of the HLA system, as a predictor of transplant success.
. It is a careful discussion!
Dear Goutham,
I will try to answer your questions one by one.
1. The molecular consequences of having polymorphic genes (A, B, C, DR, DP, DQ)within the HLA locus is that, there will be different HLA proteins expressed on the cell surface. Such differences will make HLA alleles specific for different antigens as well as TCR. For HLA class I alleles, the peptide binding groove, which is indeed, where the antigen is allocated is encoded by exons 2 and 3; whereas for HLA class II alleles is only encoded by exon 2. This mean that the vast majority of polymorphisms fall into this areas of the gene.
2. Like I said above, the mayor differences between different HLA alleles relied onto exons 2 and 3 for HLA Class I and exon 2 for HLA Class II.
3. This question is fairly the same as number 2.
4. This question isn't clear enough. If you are talking about HLA donor type or HLA antibody screening. Because it isn't the same methodology. For HLA donor typing, mainly all 6 loci are typed, depending the policy of the H&I center and for HLA antibody screening you can use both, flow cytometry or CDC to set your cross match.
Check this web site for further information: https://www.ebi.ac.uk/ipd/imgt/hla/
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