Please what is the best solvent to dissolve heme?

I have tried NaOH and NH3 so far but can't dissolve well.Thanks for the assistance. I can be contact personally on this email:[email protected]

04 January 2021 5,528 2 View

PVDF membrane degradation?

Hi, I'm performing western blots on different samples and I'm facing a some problems after eletrotrasnfer with PVDF membranes. In general, during the incubations with the primary and secondary...

18 November 2020 6,412 7 View

What to do when you found a published paper that doesn't belong to you but has your name as the author?

I found a published paper online (attached here) that bears mine and colleague's names as authors but the paper doesn't belong to us. The work was claimed to be carried out in Kenya. We are in...

22 March 2020 4,860 7 View

Do you know a (validated) instrument for the assessment of state embarassment?

I've found quite a few scales for vicarious and personal embarassibility as a personality trait so far (i. e. Kelly & Jones, 1997; Maltby & Day, 2000; Uysal et al., 2014...). As I'd like...

24 February 2020 8,142 4 View

Are Peak Areas of different silylations comparable?

If I have an analyte, that has different silylations, like 2-TMS and 3-TMS, how can I compare the peak areas? Can I simply sum them up? What if theres a different ratio of 2-TMS/3-TMS in my...

03 December 2019 273 4 View

What is a good topic for Blockchain applications in IoT?

Hello everyone, at the moment I'm looking for a topic for my bachelor thesis. I was thinking about Blockchain and Smart Contracts for cyber-physical Systems, in order to use it for Smart Factory...

23 October 2019 3,694 5 View

How to carry out western blotting for a 233 kDa protein effectively?

Hi, I want to carry out western blotting for a 233 kDa protein. I was told to use a tris-acetate gel due to the size of the protein. However, I was wandering would I be able to detect the...

25 March 2019 6,308 4 View

How to set up Coupled Displacement Boundary Conditions in COMSOL?

Dear Community, I recently switched from ANSYS Mechanical to COMSOL Multiphysics. So far I was able to transfer my models to the new platform but I yet miss one feature of ANSYS in COMSOL. I hope...

29 January 2019 4,937 3 View

SpeedVac of DNA and salt concentrations: downstream effects?

I often use a speed vacuum concentrator to concentrate my DNA prior to restriction digests, DNA ligations, NGS library preparation or target captures. Usually, my DNA is eluted in 10 mM Tris-Cl...

13 November 2018 6,049 3 View

How do I calculate NDVI images with different thresholds using Sentinel-2 image?

I want to set different thresholds for calculating the NDVI images using Sentinel-2 for the purposes of fishing out river sediments in the Dry and Wet seasons. I am using the Semi-automatic...

05 November 2018 694 2 View

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02 March 2021 3,060 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View