In Bio flocculation r temperature at temperature between 20-25˚C stable is stable temperature.If the water temperature increases bio flocs lose their floatability and sink to the bottom in the form of sludge. A number of microorganisms (i.e., bacteria, algae, fungi, and actinomycetes) are considered producers of bio flocculants .In this study, the effects of low temperature on coagulation kinetics and floc surface morphology the showed that low temperature slowed the coagulation process, as reflected by a decrease in the aggregation rate and rate constant.