How about the performance of TAMRA quencher?

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Francesc Codony

You can use the combination of FAM-Tamra, in a single qPCR, but not in multiplex PCR. In a very cheap approach I usually do it for initial evaluation of my PCR sets.

Nyuk Fong Kon

Yes. As we know MGB quencher is a non fluorescence quencher but TAMRA is a fluorescence quencher which might affect the fluorescence emission by the reporter dye in qPCR assay. I just wonder is TAMRA a good choice as a quencher?