Is there any phosphates sensor (or total phosphorus) for online monitoring of the lakes?

We need to monitor surface waters in terms of nutrients and algae content. We are building up an on-line in-situ monitoring system for early detection of cyanobacterial blooming and toxicity into...

27 February 2020 9,612 7 View

Would you be interested in hosting a Fulbright Visiting Scholar?

I would like to apply for a Fulbright Visiting Scholarship, but I need a colleague who would like to host me in a U.S. University. My research interests include Special and Inclusive Education,...

08 October 2019 3,089 3 View

Best method for dna extraction from honey ?

hello, what is the best method for extracting bacterial dna from honey? thank youu

19 February 2019 131 6 View

Does anyone have any experience with RET immunohistochemistry?

Does anyone have any experience with RET immunohistochemistry? I am interested in staining nerves in skin. Thank you

09 November 2016 7,648 2 View

Is there a way to increase the sensitivity in the "do it yourself/ready-set-go" kits?

19 October 2016 8,696 3 View

What demethylating reagent do you suggest?

I would like to demethylate a substrate which carries one aryl methoxy group and heterocycle moieties. I tried some methods by using HBr and BBr3 but they didn't give any demethylated product. My...

03 August 2016 4,711 19 View

Is anybody working on architectural spaces and/or urban places or neighbourhood projects aiming at well-being of people with dementia condition?

02 August 2016 7,382 8 View

What is the best survey/questionnaire to assess children's perceptions on weapons?

We are conducting a study on primary school children's perceptions of what constitutes a weapon in Greece.

02 July 2015 1,407 2 View

Can I use boron tribromide for the demethylation of a methoxy group on a compound which carries also a primary arylamine?

Do I have to protect the amine?

28 July 2014 3,785 3 View

Imaging extracellular membrane proteins

I want to visualize a protein which will reach the plasma membrane and localize on the extracellular space while performing live cell imaging. Is there a way to have a tag, which will only become...

03 November 2013 582 9 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

How to inerpret whether PCR went OK?

Dear friends! I am back to work after 3 years career break. Now performing a PCR, feeling like a newbie... Please, help to understand whether RT-PCR reaction "worked" or not. Which results should...

14 February 2021 3,129 8 View

Why do my polyphenol measurements fail?

In order to measure the polyphenols in broccoli, we prepared a reagent for measurement using the following procedure. 1. Extract the crushed plant with 70% methanol. 2. Mix 1 ml of the extract...

07 February 2021 845 4 View

Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View