Hi everyone!
Most of the genes, except of sex-specific, are duplicated in diploid organisms (like in mice). To get a knockout for a certain gene we need to delete an exon/s (for germ-line) or flox (for conditional/inducible) them. Anyway, in all cases we need to cross the transgenic mice to homozygosity (-/- for particular transgene) to receive a full knockout. In mice it takes a lot of time, and crossing such mice with other genotypes of interest leads to at least 2 breeds (half a year roughly) to return the strain to homozygosity for gene A with additional crossed-in gene B.
I am wondering, if there are techniques available to make the knock-out for particular gene homozygosity-independent to avoid excessive breedings. I have only the knock-in of shRNA sequence against particular gene in mind... Do you have any ideas?
Best,
Michail
Knocking out of two specific genes is called homozygous and heterozygous knockout.
Removable of the two genes responsible for homozygous and heterozygous.
It all depends which gene you're knocking out. For many genes, heterozygous 'knockouts' show a loss-of-function phenotype because of haploinsufficiency.
In genetics, "homozygous knockout" and "heterozygous knockout" refer to different types of genetic modifications or mutations in an organism's genome, typically in the context of gene function or expression. These terms describe the state of a specific gene or allele within an individual or a population.
These knockout techniques are commonly used in genetic research to study the function of specific genes. By creating homozygous knockouts, researchers can completely eliminate the function of a gene to assess its role in an organism's development, physiology, or behavior. Heterozygous knockouts are often used to study the partial loss of gene function or to mimic genetic conditions where one allele is mutated while the other remains functional.
The term "knockout" is derived from the idea that the gene's function has been "knocked out" or inactivated, allowing researchers to investigate the effects of its absence on the organism. These techniques have been instrumental in advancing our understanding of genetics and the role of specific genes in various biological processes.
l Reviewing the protocols listed here may offer further guidance in addressing this issue.
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