Is it ok to use Honey sample without hmf(Blank honey sample)for recovery studies?
Hello Ankita,
As HMF is a natural ingredient in honey, using it as a blank sample could result in measurment errors. The EU Directive (110/2001) and the Codex Alimentarius (ALINORM 01/2000) standards limit HMF to 40 mg/kg for honey produced under European conditions and 80 mg/kg for honey coming from tropical countries. Using such honeys as blank would lower your precision.
A method with HMF free sugar syrup is described here:
- https://assets.thermofisher.com/TFS-Assets/CMD/Application-Notes/AN-270-IC-Hydroxymethylfurfural-Honey-AN70488-EN.pdf
Regards,
Richard
You can use an 'artificial matrix' that you made. Crystalline Glucose and Fructose, for instance to make a synthetic honey. Thus, you would have a 'blank' matrix for recovery.
Dear Richard Odemer
I was randomly testing honey samples and i found one honey brand with zero/nil HMF values. So i used it as a blank honey sample(as it contain honey matrix but no HMF).
is it going to affect my Method validation?
Dear Ankita Sood ,
I see. In that case, I guess it should not be a problem. You could test a blank sugar matrix additionally...
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