Dissolution study on nano co-crystals ?

Hi, SO I prepared nano co-crystals and I have done a dissolution study on them, the thing is I'm getting 130% of my co crystals at the end of dissolution which doesn't make sense !! I have...

26 November 2020 8,816 4 View

Why we can't apply energy or forcefully dissolve lipids when preparing liposomes by thin-film rehydration?

I have read that we can't apply energy or forcefully dissolve lipids when preparing liposomes by thin-film rehydration? I can't remember why or find the source of information, any help?

11 October 2020 3,950 3 View

Difference between polymeric nanoparticles and Liposomes?

What is the difference between PLGA PEG nanoparticles and Liposomes? any recommended papers or reviews that explain the pros and cons of each formulation ??

06 September 2020 6,341 3 View

How to identify the appropriate sites and design a passive seismic survey in order to support the site characterisation?

In the case of overpopulated cities, how I can select the appropriate sites to do some field measurements "Passive Seismic Surveys"? How about the design of the survey in order to study the soil...

04 August 2020 5,253 4 View

How to include or insert the value of LSD (limit of standard deviation ) in an excel graph ?

Please, can a colleges tell me how to include or insert the value of LSD (limit of standard deviation ) of the statistical analysis in an excel graph both (column and line graph)?

04 June 2020 3,835 3 View

Are there any theory/ framework/ model for online teaching?

Are there any model or theory concerning online teaching/learning esecially in EFL context. Are there any model also for emergency remote teaching ?

01 June 2020 3,687 16 View

How can we write research about using technology in teaching while we don't have even stable electricity and internet connection?

It is important for researhers to write about new and up to date topics for example using technology in teaching. In Yemen such topic is difficult to be really tackled because even electricity and...

02 May 2020 2,501 7 View

No title

I need help regarding the solubility of polymeric metal complexes and how to prepare their homogeneous layer on ITO or FTO substrate. i have to prepare working electrode for CO2 reduction

23 June 2018 7,617 2 View

Can I design a survey asking the sample about a technology that they might never actually used?

16 June 2018 5,923 2 View

Please need to help ! what is the suitable method of PCR type (PCR-RFLP, RT-PCR or PCR) with next genes ; STAT4,TRAF1,C5,PTPN22 and SIRT-1??

needing to search about associated genes with RA

19 February 2018 9,773 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Can any one provide information about medicine, epidemic and critical issues or it is mandatory to be an expert ?

Due to the pandemic of coronavirus, the most source that the people depend on was social media. There is no guaranteed solution that is biased from political opinion. An example of that the...

01 February 2021 7,567 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View