Hi-- I'd like to extract CTLs from primary tumor and test proliferation profiles. any experience with harvesting viable t cells from primary tumors?

More Justin Markel's questions See All

Error in solve.default(V, tol = .Machine$double.eps) : Lapack routine dgesv: system is exactly singular: U[28,28] = 0 how to resolve?

hello everyone, Anyone can help me fix that error that occurred when I was running PGLS model in caper package .

21 February 2024 3,475 0 View

Is there a preferred method of reporting GPS coordinates in field biology?

Does it matter how GPS coordinates are reported in field biology - as in, is there a general preference between reporting GPS data using sexagesimal degrees (Degrees, Minutes, Seconds) or decimal...

12 January 2024 9,045 2 View

What methods can be done to ensure that laboratory workers follow proper quality control procedures?

One of the factors that can affect the quality control of the laboratory is the laboratory workers. However, some laboratory workers do not follow the procedures for various reasons. What methods...

03 September 2023 3,548 3 View

What are good approaches to research etymologies?

I am considering doing some research on the etymological origins of certain words related to student government and student leadership, particularly words such as "student", "pupil", etc., to...

08 August 2023 4,539 6 View

How do you fix a stuck pump on a Biotage Isolera?

I am currently trying to just prime my Biotage Isolera but it keeps giving me an error saying pump A is stuck. I tried cleaning the check valve to see if that will clean anything, it did not....

22 June 2023 569 2 View

Are academic book reviews worth it?

Do academic hiring committees consider book reviews? I enjoy doing book reviews because I get free books, small publications, and it allows me to stay up to date on recent scholarship in my...

21 May 2023 7,997 9 View

Does anyone know how, after importing a correlation matrix in R, I can specify the # of observations?

22 February 2023 9,972 1 View

How difficult was it for you to obtain a tenure-track position?

What was your experience searching for academic jobs for the first time? Were you able to obtain a tenure-track position? How many jobs did you apply to, where did you apply, and what was the...

14 January 2023 4,928 5 View

Why do we have to do a seismic model before carrying out inversion process?

A geophysical model is obtained before inversion process.

11 December 2022 4,645 3 View

What is the significance of carrying out wavelet extraction during seismic data processing petrophysical analysis?

The above question is part of the workflow for inversion process using 3D seismic wave data

11 December 2022 8,394 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Colt Egelston

Lots! Any specific question? Mouse or human?

Justin Markel

Mouse!

Suliman Abdallah Alsagaby

What you can do is isolate the PBMCs from whole blood and then conduct a positive isolation of the CTLs using Dynabeads coated with monoclonal Ab against mouse CTLs CD marker. In human CTLs it is CD8.

Hope this help.