Justin Markel

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Questions related from Justin Markel

Any equipment that can help mechanically dissociate strong sticky organic pellets?

Organic matter: PLGA Anything that can help grind it to a powder?

06 February 2017 8,568 2 View

What can I use to dissolve PLGA nanospheres (< 3 microns in D) without affecting the integrity of the protein within?

These PLGA nanospheres are extremely densely packed, and do not dissolve well in solution. I have already tried DMSO and release solutions such as SDS and 0.05M NaOH. The protein encapsulated in...

03 January 2017 836 3 View

Hi-- I'd like to extract CTLs from primary tumor and test proliferation profiles. any experience with harvesting viable t cells from primary tumors?

Harvesting viable immune cells from primary tumors in mouse model

26 October 2016 5,105 3 View

Any information on how to measure the elution of a cytokine from a nano sphere in vitro?

So far, we have put the loaded spheres in PBS and measured concentration via ELISA at different time points (one sample per time point). Any idea if spectrophotometry could be used for this? If...

01 September 2016 4,207 3 View