I´m working with liver biopsies from pacientes for my master´s project. My 260/280 values on NanoDrop were 2.0-1.9 but the 260/230 values of four pacients were between 1.71 and 1.46. I will use them to RT-qPCR
Hi, you can re-purify RNA, refer to this previous post:
https://www.researchgate.net/post/RNA_re-purification_Protocol
However, depending on your qPCR reagents and method (TaqMan or SYBR), your A260/A230 is not that bad; it should work fine (in my humble opinion, because i once tried with A260/A230 of 0.6 for SYBR qPCR, and even the housekeeping Ct values does not shift), especially if the RNA to cDNA conversion process is efficient. Good luck!
Yes, use it for qPCR but verify RNA integrity and include controls—cleanup only if amplification fails.
You can go ahead and convert to cDNA and proceed with RT-qPCR. If you do not get desirable results, repurify, which will result in significant amounts of RNA loss, depending on the purification protocol. We have repurified RNA using the phenol: chloroform method with acceptable results. Alternatively, you can also use commercial kits to repurify RNA.
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