Ahmad Hafiz Murtadha

3 Questions 11 Answers 0 Followers

Questions related from Ahmad Hafiz Murtadha

Calcium Phosphate Transfection for MCF10A?

Pardon me, but it would be great if anyone who had successfully done calcium phosphate transfection on MCF10A cell line can share their protocol/recipes. I would want to compare with mine. I've...

09 September 2021 8,596 1 View

Can MDA-MB-231 Spheroid Form on Agarose Coated Plate?

Hi, I wanted to ask if anyone ever managed to create a regular shaped (for drug testing evaluation) 3D spheroid for MDA-MB-231 on agarose coated 96-well plate without any addition of...

30 May 2018 6,761 3 View

What is the most prominent HLA Class 1 allele target for qPCR analysis in MCF7?

Hi, I wanted to detect changes in MHC Class 1 expression using qPCR in MCF7, but MHC Class 1 is categorized as classical (HLA-A, B, C) and non-classical (HLA-E,F and G) and some of it are highly...

14 May 2018 1,320 5 View