LiFePO4 DRX peaks?

05 October 2020 8,919 3 View

How to estimate charge transfer and polarization resistance in a battery?

Hi. I have performed many EIS experiments using a cylindrical battery at different temperatures and state of charge. Some results (near SOC=0) are as the attached figure. How can I find the...

08 July 2019 1,746 2 View

Are there different types of Dansyl Chloride?

I was using Sigma Aldrich Dansyl Chloride (03641) suitable for HPLC derivatization on a method for quantification of hormones, but then I changed it for product (39220) for fluorescence and now I...

24 June 2019 1,484 3 View

E.coli cryopreservation for routine subloning at -20?

I would like to know if there is any protocol or personal experience to cryopreserve chemically competent E.coli cells (for routine subloning) at -20C. The only ultra-freezer that I have access is...

20 December 2018 3,718 3 View

E.coli cryopreservation for routine subloning at -20?

I would like to know if there is any protocol or personal experience to cryopreserve chemically competent E.coli cells (for routine subloning) at -20C. The only ultra-freezer that I have access is...

11 December 2018 1,592 3 View

Loss of internal standard in a LC ESI MS/MS protocol for Gliclazide?

We are performing an LC ESI MS/MS protocol to quantify Gliclazide on plasma samples. We have noticed that the internal standard (Gliclazide D4) has a very high variation compared with gliclazide....

22 July 2018 8,936 4 View

Which is the best way (protocol) to dissolve 4-Hydroxytamoxifen?

Hi everybody, I was wondering which is the best way (protocol) to dissolve 4-Hydroxytamoxifen. I would also like to know how can I store the reagent to keep it stable and useful for future...

19 June 2018 1,757 4 View

What is half-life of mRNA of Tbet, AHR, and RORc?

01 February 2018 3,654 0 View

Does anybody know a good (an not too expensive) infrared thermal camera to measure mouse temperature?

Hi, we would like to have a more visual reference of what is happening in our mice after cold-induced browning. We are measuring temperature using a rectal probe thermometer, but It would be great...

28 November 2017 7,897 3 View

Do anyone Know if there is some procedure to perform Lumsdaine and Papel test for unit roots breaks in stata or gretl?

In RATS you perform LPUNIT procedure. I want to know if I can do in other statistical software

27 September 2017 4,208 2 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View