Dear Guy
Please, see the following links, I hope will be useful for you.
https://www.youtube.com/watch?v=wXiiTW3pflM
and:
https://www.youtube.com/watch?v=U2-5ukpKg_Q
Good Luck
Dear Guy,
It should be possible to cast gradient agarose gels in a similar way I casted my big slab SDS PAGE gels. This set up is described in my paper (Electrophoresis 1994, 15, 1014-1020) which scan is included on my Research Gate page.
To do this one would need to cast the gels vertically in a closed cassette. The cassette and the gradient maker should be kept warm to prevent condensation of the agarose. To ensure ideal gradient the casting should start by pumping agarose (at its highest concentration) first at the bottom of the cassette while constantly keeping the long syringe needle just above the its surface.
I would be glad to discuss with you by Skype how to set up a casting device for this kind of gels.
With best regards,
Apolinary
Casting the gradient is simple (just add some 10% glycerol to the gel mixture of higher concentration if need be), but what would you like to achieve? Gradients in the gel matrix work if the gel is restricting, that is, the pore size is of similar magnitude as the sample molecules. Acrylamide is a restricting gel for both proteins and nucleic acids. Agarose, with a pore size of several hundred nm, is restricting only for very large particles, like virus or chromosomes.
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