I have pLys cells, BL21 gold and pRIPL. And, if I can, what is the best protocol of induction? I have an expression vector with a T7 promoter, a pUC origin and a amp bacterial selection. I have no tags.
In my past lab, for a protein form S. Salar we used BL21 (DE3). I think that you can use too
Thank you. And what kind of expression vector have you used? The protein that you have expressed needed post-translational modifications?
pET22b+, and no post-translational modifications :)
ok, thank you :)
if you want more information, you can contact me in private
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