Carmela Consiglio

2 Questions 4 Answers 0 Followers

Questions related from Carmela Consiglio

Hi. I have to express a protein which is from an Antarctic fish. Can I use plys cells from e.coli?

I have pLys cells, BL21 gold and pRIPL. And, if I can, what is the best protocol of induction? I have an expression vector with a T7 promoter, a pUC origin and a amp bacterial selection. I have no...

21 March 2017 9,949 5 View

Have you ever these cloning problems?

Have you ever cloned a gram negative gene in a gram negative bacteria?I'm cloning two genes of a gram negative bacteria (thermophilus) in E.coli but after the cloning (positive pcr) and the...

18 March 2016 8,233 6 View