How to estimate total electron content using GPS data (with MATLAB)?

06 January 2021 1,110 2 View

How can I find the area of contour with magnitude less than specific number?

Hello everyone I have results from fluent and I want to determine area with magnitude less than specific number for example for velocity or TKE. and also how can I determine Max and min diameter...

02 November 2020 5,863 3 View

Why residuals stay at the same range in vof model in Fluent and it doesn't converge?

hi I'm modeling a 3d channel with vof , k-epsilon RNG with non-equilibrium wall functions , for bc I have velocity inlet, pressure outlet and for top of the domain I have used pressure outlet...

21 August 2020 5,926 7 View

How I can obtain the cartesian coordinates of a complex without single crystals for gaussian calculations?

When the single crystals of complex are not formed, how we can obtain cartesian coordinates for Gaussian calculations? We want to predict kind of interaction or coordination of manganese to amino...

18 August 2020 8,146 3 View

I am confused about the editor's request (I had submitted a manuscript and then major revision was requested). Can anybody help me?

I had submitted a manuscript to a journal. After a while, they requested major revisions. The end of their request, they mentioned that: "You will be unable to make your revisions on the...

09 August 2020 5,436 7 View

Is it possible to extract RNA and protein from 96-well plates?

I am doing a knockdown test in 96 well plates and then I need to extract RNA and protein from my cells. I'm not sure that the number of cells in 96 well plates is enough to these extractions....

12 June 2019 5,459 3 View

In SDS-PAGE , how possible to use freez-dry samples for loading in gel electrophoresis?

I freeze fungal mycelia of mushroom. In SDS-PAGE of total protein ,If I freeze mycelium I can used this sample? How should I prepare mycelium for electrophoresis? How should I make it for...

01 October 2018 7,971 5 View

What is the extinction coefficient of pyrogallol at 430nm for calculation of PPO(polyphenol oxidase) activity?

I performed the polyphenol oxidase activity assay from certain mycelia extracts using pyrogallol as susbtrate. I have the absorbance values but am facing the problem in determining the enzyme...

05 August 2018 172 1 View

Many thanks for all your help?

This question has been deleted.

30 March 2018 7,374 5 View

Is there any software to determine the number of codewords with fixed GC-content in DNA codes?

If so where can I find it?

03 January 2014 9,116 8 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View