Dear Hassani,

106 per 1mL of Trizol would be fine but it depends on your cell line and the downstream applications.

Best,

Hamadi.

Which cells, which species?

A typical mammalian cell contains 10–30 pg total RNA.

See also:

https://www.researchgate.net/post/what_is_the_maximum_number_of_cells_that_I_can_used_for_RNA_isolation

thanks hamadi.

sabine, its mda-mb-231 breast cancer cell line.it must be the same as you just said, doesnt it?

Cells seeded on a 35mm plate shall be more than enough with 1ml Trizol.