I need some details for starting my project in lab.
I've designed media for CHO cells, without Glutamine! But when they're in large scale, the cells were damaged and decreased their viability. What's your refer ?
14 January 2024 2,503 2 View
How can I find "PCR markers" and "suitable antibody to Flow cytometry" of C2C12 cell line? Is there any web server to find this data?
02 December 2023 3,248 0 View
Can someone provide me with the earthquake repositioning code with Python?
29 September 2023 6,469 3 View
Hi ResearchGate community, I have been trying to learn more about the optical differences between block-based real-time PCR machines like ABI StepOne versus rotor-based machines such as MIC or...
22 July 2023 964 6 View
hello, I have a major question that i would really appreciate if anyone could make me clear. In a project I am going to transfect BMSCs with a plasmid by lipofectamine 2000 reagent. then the...
06 June 2023 9,768 1 View
I want to use an epilepsy database ( CHB-MIT Scalp EEG Database) and the samples have some bipolar channels like FP1-F7, F7-T7, T7-P7, P7-O1, FP1-F3, etc. We can define monopolar channels in...
12 July 2022 3,546 0 View
I dont mean bacterial cellulose(the cellulose which produce by bacteria like G.xylinus ) or the cellulose fibril that present in paper mill activated sludge, i mean a protocol for extracting the...
02 July 2022 6,182 2 View
Is there any free available tool or even demo that gets the MRI image and creates the contouring of different organs? I need to just test some few cases. Thanks.
07 March 2022 1,689 0 View
Hello everyone I am working on a human cell-line of Oligodendrocyte Precursor cell (OPCs) called MHB. Results of my immunocytochemistry experiments showed that these cells express NG2, and don’t...
15 December 2021 3,208 1 View
I have been searching the publications reporting STR markers in the genomic neighborhood of my interest, but I wonder if there might be a more straightforward approach to it. Also within the UCSC...
23 November 2021 8,236 4 View
I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...
16 July 2024 5,031 1 View
Can the kit from Invitrogen™ LIVE/DEAD™ Cell Imaging Kit (488/570) be used on fixed cells on a scaffold?
08 July 2024 7,914 3 View
"What is the most accurate method and model for evaluating the effective mechanical and viscoelastic properties due to the inclusion of multiple types of particles in a viscous material, and which...
16 June 2024 9,139 1 View
Suppose we have dried MXene slurry (Ti3C2Tx), then the combination of which solvent (in which this dried MXene slurry powder will be mixed, subsequently forming the "ink") and corresponding...
13 June 2024 5,763 0 View
I have been trying to contact them for about 2 weeks now and I have received no response. I am wondering if they are still active. If not, could anyone suggest a similar foundry service to do some...
12 May 2024 2,100 0 View
hello, I have a scaffold with three layers, two synthetic polymer layers and one central layer made of ECM gel seeded with SMCs. After in vivo culture, how can I determine through Masson's...
05 May 2024 2,297 0 View
Dear connections I want to perform scaffold hopping of some molecule to get novelty on the reported compound can you suggest me the best software for the same. your support will be appreciated...
01 May 2024 5,864 1 View
can you suggest any schrodinger interface for scaffold hopping
01 May 2024 1,351 0 View
I have a reference and hit molecules. I need to compare and differentiate the scaffolds present in this hit molecules. my aim is to identify which scaffold that plays a significant role in AD.
01 May 2024 6,562 0 View
I'm trying to incorporate curcumin in my polymer scaffold. 1. Is idea of soaking the 'scaffolds' in the curcumin solution is good? 2. will there not be any wastage of curcumin while...
01 April 2024 387 2 View
