Do you know any company in the US to buy mCherry-red tagged SUM159? How about mCherry plasmid?

25 October 2023 0 884 Report

Hello!

I wanted to buy mCherry SUM159. Our supplier won't work anymore and I couldn't find any websites to order it. Would you please let me know if you know someone to help me with that?

Thank you

Badges
Science topic

Similar topics
Chemistry
Chemicals
Solvents

More Parisa Taklifi's questions See All

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

How can I quantify MDEA (N-Methyldiethanolamine) with a GC-FID?

I have a mixture of glycol, water, MDEA, and piperazine. I want to analyze it with GC-FID.

15 July 2024 9,652 3 View

How can I quantify MDEA (N-Methyldiethanolamine) with a GC-FID?

I have a mixture of glycol, water, MDEA, and piperazine which I want to analyze with GC.

15 July 2024 2,181 10 View

How to download all 3D structures of IMPPAT database?

Hello I want to know is there any way to download all 3d structures of natural products of IMPPAT database?

14 June 2024 7,298 1 View

Why my PCR buffer is not working for one gene (while it was worked well for other gene)?

We prepared a PCR buffer and assessed it with approximately 12 primer pairs, all of which resulted in successful amplification. However, there was a specific gene region that failed to amplify....

13 June 2024 548 3 View

Is there any replacement for primer blast (NCBI) to see if our primers are specifically designed for our desire PCR product?

when I insert my primers in primer blast site and push the button "get primers" I just reach different errors and I don't know what the problem is!

22 April 2024 8,001 5 View

Is there anyone who has experience working with Hypoxic Chamber (Glove Box-COy)?

I want to culture Huvecs cells under hypoxic condition (hypoxic chamber), Does anyone know the cell culture and treatment process?

15 February 2024 6,732 0 View

Microfluidic chip- how to prevent hydrogel peeling and retraction?

Hello! I inject 1 mg/ml Collagen + matrigel mixture in one channel of my device and 2mg/ml collagen in the adjacent channel. Two channels are separated by microposts. I somehow see hydrogel...

11 February 2024 8,181 1 View

Hi guys, Has anyone worked on fibroblasts?

I'm looking for the specific marker of lung fibroblast in systemic sclerosis.

25 December 2023 4,969 0 View

Dear researchers,. Have any of you successfully synthesized ZIF-67 in the laboratory?

Dear researchers, I am seeking assistance in synthesizing ZIF-67 for my laboratory-based PhD thesis. Have any of you successfully synthesized ZIF-67 using any of the following methods: 1)...

18 December 2023 8,152 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View