If the drug you use is effective to cancer organoids, how much longer would you wait for after adding the drug to the culture system in order to better match clinical data.
We are doing experiments in different type of tumor spheroids. Depending on used cancer cell type to make them, and also on the ratio of cancer cells with different type of cells (if you make multicellular spheroid), they can grow/behave differently. Usually we add our compounds when we have spheroids with diameter of 300-400 um, and replace the medium containing compounds every second day. We do these experiments from 10 to 20 days (depending on the control, until it begins to disintegrate).
Hi - this paper will give you some idea (https://www.nature.com/articles/s41467-018-05190-9) but this relates to esophageal adenocarcinoma derived organoids.
Bottom line is the organoids either form or not, then you continuously grow/passage them for a while - then you use a trypsinised organoid suspension to seed a 384-w plate for drug screening. Your question re: ' to better match clinical data' is a tricky one - my opinion is organoids are just another assay model, which won't necessarily predict a clinical response. There's no vascularisation, for eg.... 3D culture is very popular at the moment but take it with a 'grain of salt':
https://www.nature.com/articles/nrd.2018.99
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