Hi everyone. I would like to use the following PCR primer 5' AAGACCGCGACCTTPATGCC 3'. what Does P stand for?

03 March 2017 1 7K Report

The primer is from the publication Hurt et al 2001 : "Simultaneous Recovery of RNA and DNA from Soils and Sediments" and is used to amplify the eubacterial glutamine synthetase.

Jillian M. Couto

Fabian it appears to be a degenerate base, but it doesn't use the standard IUPAC notation. Have they provided a definition in the paper?

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