Fabien Cholet

3 Questions 3 Answers 0 Followers

Questions related from Fabien Cholet

RNA Inegrity Number (Agilent RIN) anomaly?

Hi all, I've been extracting microbial RNA from environmental samples (mud) and ran my extraction on the Bioanalyser to check the integrity. I've obtained surprising results : the sample with a...

10 October 2018 7,739 0 View

Is there an effect of well position on ct in qPCR?

Hi, I often hear (and say) that tubes should be put at the center of the PCR machine for a better reaction performance. I was wondering if the same applies for qPCR and therefore if there is an...

07 July 2017 9,177 5 View

Hi everyone. I would like to use the following PCR primer 5' AAGACCGCGACCTTPATGCC 3'. what Does P stand for?

The primer is from the publication Hurt et al 2001 : "Simultaneous Recovery of RNA and DNA from Soils and Sediments" and is used to amplify the eubacterial glutamine synthetase.

03 March 2017 7,371 1 View