Hi everyone, I am having a challenge to punch the mouse hippocampus. Does anybody have experience in this area and can help?

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Xavier De Jaeger

Hi,

could you specify what you mean by punch ? If you mean dissect here you have nice explanation: https://www.youtube.com/watch?v=Upf15CB29V4

Galina Bursow

Oh, thanks, but I need to separate (punch) only a tiny part of hippocampus. I'm going to use special punch needle for it.

Alberto Sepulveda Rodriguez

Do you mean in vivo from a whole animal? After dissection as summarized above?

What specific region and layer are you targetting?

I need to punch the injected area in hipp from frozen brain. I would do it in cryo microtome.