AT8 has a dual epitope at pS202 and pT205 on tau. To my knowledge there is no rabbit polyclonal antibody that reproduce exactly the AT8 epitope. The closest is an anti-pT205, which will reflect more the AT8 signal than an anti-pS202. So when we want to do IHC with a polyclonal to get an AT8-like signal, we use a anti-pT205. There are many of them available commercially:

http://www.alzforum.org/antibodies/search?category[616]=Tau

We have used the Invitrogen (44738G) with success. You can see its signal on WB and compare it to AT8 on this paper from our lab:

http://www.ncbi.nlm.nih.gov/pubmed/24788298

Hello

I agree with Emmanuel Planel , gave you a good advice

Good Luck

HI,

pT205 is an excellent option. If you wish to broaden you can also try pS199, pT212 and pS214. We have obtained comparable results with antibodies against these epitopes from Invitrogen. 

http://www.sciencedirect.com/science/article/pii/S0197458010004604

I thank all of you for the excellent answer!

I can recommend the following: 10F8 mouse anti-pTauS202 available from abcam or directly from aj roboscreen. 

Unfortunately not from rabbit but with great specificity without any background in IHC.

Hi Galina,

I'm not sure if you're still looking into this, but StressMarq has a new rabbit anti-pSer202/ pThr205 tau antibody with free sample sizes are available to test:

https://www.stressmarq.com/products/antibodies/tau-antibody-pser202-pthr205-smc-601/

Hi Patricia, its a good news:-), Thank you a lot for this information!