I want to study the physiology of the larvae of a moth, and I must cut the cross section of the body of the larvae, and see the fatty granules of the body. Dear researchers, What is the simple way to create a transverse section of the larvae?
1. Are you wanting to see the fat body cells, or the lipid droplets within the cells?
2. What type of microscopy will you employ (brightfield, fluorescence?)
3. Which stain did you plan to use?
I would maybe suggest fixing the larvae (e.g. in 4% fresh paraformaldehyde) and embedding in paraffin (or similar), then sectioning and staining. You can visualize fat cell morphology with Nile Red or Tubulin antibody (both fluorescence stains), or probably even hematoxylin & eosin (brightfield).