Hi, currently I am working on DNA extraction of bamboo leaves.What is the suitable concentration of DNA should I get for PCR process?

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Hi, currently I am working on DNA extraction of bamboo leaves. This is the picture of the DNA extraction result.

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Rajesh Kumar Meher

Hi Jerlyn,

you need atleast 30-40 ng conc. of DNA per microliter for good PCR result.

Jerlyn Joan Jenius

Thank you Rajesh for the answer. Appreciate it very much

Giorgia Pertile

It depends on what kind of gene you need to amplify. For example, if you need to amplify a portion of the rDNA (it is present in many copies) you don't need much DNA, but if you need to amplify a functional gene, you need more. In case you are analyzing for the first time you convince yourself to try the same sample at various DNA concentrations.

That really helpful. Thank you Giorgia Pertile