I am aiming to do isolation of D.salina by flow cytometry.
I am not sure if i compulsory use nile red for this purpose because some articles show the NR is used for lipid secreening and nobody work to isolate D.salina using this techniqe but i think this attachment will help to declare this aim but why they use nile red to secreening the lipids althought their aim was to select dunaliella salina strains.
i have colored sample because of beta carotene produced by D.salina so i think i have to use the wavelength of beta carotene only for sorting the cells.
am i right?
Have i to use a acontrol ? and what it will be ?
I think the following below links may help you in your analysis:
· P.Hyka, S.Lickova, P.Přibyl, K.Melzoch, K.Kovar.Flow cytometry for the development of biotechnological processes with microalgae. 31( 1,) ; 2013, Pages 2-16
· Yilancioglu K, Cokol M, Pastirmaci I, Erman B, Cetiner S. Oxidative Stress Is a Mediator for Increased Lipid Accumulation in a Newly Isolated Dunaliella salinaStrain. Campbell DA, ed. PLoS ONE. 2014;9(3):e91957.
· Héctor Mendoza.Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains.11(4);2008
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