Prema Mondal

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Questions related from Prema Mondal

HeLa cells have been detaching after heat stress. Can someone please suggest some way to keep the cells adhered during heat stress and recovery?

I have been growing HeLa to 90-100% confluency in 12 well dish, prior to subjecting them to heat stress at 43 degrees Celsius for 1.5hrs in a water bath. Unlike HEK293T, the HeLa cells lose...

17 April 2024 7,309 0 View

Hi, can anyone suggest anti-ubiquitin antibody that is specific for mono-ubiquitination and does detect not poly-ubiquitination or free Ubiquitin?

In our in-vivo assays, we see a modified band which we know is a mono-ubiquitin band. We still want to confirm with a mono-ubiquitin specific antibody, if the same is available commercially.

20 November 2023 7,354 0 View