Hi all regarding free living amoebae what is the best current media for cultivation?

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I have get this error when i calculated the geometrical optimization for prco3, it takes 12 hours until gives this message in the outputfile?

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Yukifumi Nawa

Hi, Mohamed, I am not a specialist of in vitro cultivation of free living amoebae. Please try to contact Dr. Tatsuru Hara who is a real expert in this area.

Mohamed A El-Malky

Konitshwa, Domo Aregato Nawa Sensi

Thanks a lot

Stefan Geisen

Hi Mohamed,

There is not a best medium,it depends on what you want-they all have there preferred medium and prefer specific food.

For a great overview see the papers from Alexey Smirnov, for instance

Guide to the methods of study and identification of soil gymnamoebae

https://www.google.de/url?sa=t&source=web&rct=j&url=http://protistology.ifmo.ru/num3_3/smirnov.pdf&ved=0ahUKEwiG2rKckP7PAhVJYJoKHR0pCjYQFggbMAA&usg=AFQjCNGe9CNH5eijdzfJ9Tv9Ho3PaWIHDQ&sig2=pkjRU8hb5kXtrNxfCwt3MQ