09 July 2017 0 2K Report

I have used DMPC cholesterol so far but I would preper POPC/cholesterol

More Panagiotis Fotakis's questions See All
please if you could suggest papers for time as an indicator of an attacking transition in football?

timing and its importance in a successful attacking transition in football

01 March 2024 2,973 0 View

Is clustering nodes on existing shards an efficient approach or is it a security breach?

Greetings, I would like to know whether it is possible to enhance efficiency and decrease latency by clustering nodes according to their longitude and longitude coordinates on existing shards...

28 December 2023 4,006 1 View

Is there any reliable treatment method for destroying a collector adsorbed inthe surface of concentrate?

I am running some flotation experiments with limited sample availability. One alternative i have is to recombine the products, destroy the chemicals and use it again as feed in other flotation...

08 March 2023 2,869 2 View

Statistical Analysis of RT-qPCR data, what test do I select?

I have done my qPCR experiments and gave me some results, I used the DDCt method and I calculated the 2^(-DDCt), I transformed my data in base 10 logarithm and separated my samples between control...

21 December 2022 5,358 4 View

How to deterimne the template amount of cDNA for qPCR?

I am currently using the Quanti-Nova SYBR Green PCR Kit from Qiagen and I would like to know how much cDNA (ng) will be sufficient for amplification of my genes of interest (BMP2, 6 and SMAD6)....

06 October 2022 2,667 3 View

Could you provide solutions about the variance of seawater hardness when adding NaHCO3?

Phreeqc is a software that can be used to calculate hardness in water. There are many factors that can affect the hardness of water, including the type of minerals present, the pH, and the...

05 May 2022 6,731 0 View

Can someone provide some perspective situations of the latest blockchain consensus algorithm except the hotstuff and sbft?

Hello researchers, I am interested to find new alternatives to blockchain consensus algorithms that existed so far in academia since max 2 years describing new methods of how a scalable...

14 February 2022 5,520 3 View

Could you suggest solution processable electrodes (source, drain and gate) for OFETs fabrication?

organic field effect transistors OFETs for biosensors, chemical and optical sensors.

04 November 2021 7,235 2 View

How to simulate a machine with specific capacity in Arena Simulation?

Hello everyone, I am new in Arena Simulation Software, and I would like to know how I can simulate in a Process, a machine that has a specific capacity, like 1500 kg per hour. I think that...

08 June 2021 7,341 3 View

Does anyone have experience with the absorption of bacteriocins from LAB, on different commonly used filter membranes?

I have encountered a potential problem with filter sterilization of supernatants from Enterococcus strains, which according to PCR data, are hosts of several multiple bacteriocin genes, but show...

31 March 2021 4,190 4 View

Similar questions and discussions
Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

How to dispose off lipids waste?

I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...

25 July 2024 637 5 View

Extraction of phaspholipid from soy lecithin?

I am doing an extraction of Phospholipid from soya lecithin but the maximum concentration I am getting is 29.5 % but I need to get it around 50% in HPLC. I am extraction from Acetone and EtOH...

24 July 2024 6,474 4 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 5 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?

18 July 2024 9,831 0 View

My article: The Extended Pedersen hypothesis can you correct the the name of first Author?

In My article: The Extended Pedersen hypothesis (published, February 1988 Clinical Physiology and Biochemistry 6(2):68-73,) there is mistake of the name of first Author: it is not Catherine...

11 July 2024 1,801 2 View

Is it possible to generate a (semi-)permanent "transcription bubble" within a replicable plasmid?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...

07 July 2024 8,275 5 View

How to dilute essential oil from plants to assess the biological activity?

I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...

04 July 2024 8,100 4 View

Phospholipids storage conditions?

What is the protocol for storing phospholipids in chloroform solution? Should I divide the contents of a bottle into aliquots to prevent melting each time I take it out for an experiment from...

27 June 2024 2,138 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close