Hi all, can anyone suggest how long PFA storage of lung tissue sample would be the best before making the block for sectioning?

More Junayed Nayeem's questions See All

Hi all, Can anyone provide a reliable Immunohistochemistry protocol from frozen sections?

Any good paper reference will be highly appreciated! Many Thanks in Advance!

11 December 2023 2,459 2 View

Which is the best software or programming language (user friendly) for analysing data?

I have a basic knowledge on Python, R and MATLAB in analysing data. Among those, which is the best and user friendly? Which one should I learn deeply to analyse data?N:B: I want to learn data...

20 February 2021 4,381 15 View

I am looking for ICRG data, can someone help me with this?

For a research purpose i need this data set

05 June 2020 3,376 20 View

How do I calculate the surface area in terms standard deviation and correlation length?

The goal of this project is to model a thin film capacitor where black silicon is to be used as the parallel plates. My initial task is to look at different samples of Black silicon surfaces and...

02 May 2019 4,274 1 View

What are some effective ways to present qualitative data visually? Anyone can suggest good resources for this?

Often I find it challenging to represent qualitative data (interview/ observation/ image analysis) graphically. Describing in written words are okay but I think its more effective if I can present...

31 January 2019 9,946 7 View

I would to transform KML (digital elevation) file into 2D grid matrix in Matlab. Can any one help me in this regard please?

KML files usually relates to digital elevation data for a terrain.

03 December 2018 3,285 1 View

Where can I get a generic model of wind turbine i.e Vestas for PSCAD , as my simulation would be on VSC HVDC based wind turbine and Fault calculation?

To go on real time simulation, i am supposed to work with perfect model of wind turbines type 4 for offshore, and VSC based HVDC. If it's not possible, would you give me some suggestions how...

24 October 2018 8,694 2 View

Can anyone suggest that what is the doubling time for PC-3 cells?

I am new in culturing the PC-3 cells. I have cultured in RPMI-1640 medium supplemented with 10% FBS. I have plated 200,000 cells per well of 6- well plate.

22 October 2018 4,254 1 View

How can I start my work in my research paper for VSC HVDC, with Wind Power Grid penetration and Grid Stability?

I am planning to simulate it in PSCAD, with VSC converters HVDC. Also grid penetration of wind firms and stability should be checked with other fossil fuel generation to the Grid. Would you...

18 September 2018 4,511 3 View

What are the journals that publish architectural and urban design related researches?

Need to know the list of journals that publish architectural and urban design based research publications.

06 May 2018 2,331 7 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

checklust of the vaSCULAR FLORA NATIVE TO ITALY?

a second update to the checklust of the vaSCULAR FLORA NATIVE TO ITALY

24 July 2024 2,403 0 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View