I need hesperidin conc of 100,200,300,400 microM.
I am doing following -
Made 100 mM hesperidin in DMSO.
Then took 1 micro L (100mM hesperidin) + 1000 micro L media i.e 100 microM.
2microL(100mM hesperidin) + 1000 micro L media i.e 200 microM
3microL(100mM hesperidin) + 1000 micro L media i.e 300 microM
and so on........(working conc.)
The problem I am facing is - hesperidin gets precipitated when I put 100 microL of working conc. in a 96 well plate. How can I solve this precipitation problem?
This is solubility realted issue. Please tried to find out solubilty of hesperidin first.
Hi Chandra,, I made 10 mM solution in DMSO, then I took 50 µL and add 950 µL BSA to make 500 µM of hesperidin solution, and made serial concentration from it.
Treat the Hesperidin with cold butenol, keep it overnight at 4oc, now you start your work, it will never precipitate. You can reduce the quantity of BSA.
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