Hello, I am curious if anybody has insight into doing a helper phage rescue with either hyperphage or M13ko7-d3 (helper phage which are deficient in the pIII protein). [CLARIFICATION: I want to make more helper phage not rescue a different phagemid from a cell]. My understanding is that you have to add the pIII protein to the packaging cells either by using cells which have the pIII protein in the genome or by adding a low copy plasmid which directs pIII synthesis to the periplasm. I was wondering if anybody had specific bacterial strains or vectors which they knew were good for this. I can't find much published info on this (seems like something of secret recipe) so any insights would be appreciated.