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Hello, I have a curious situation. I have been biopanning against antigen. My phagemid vector has an amber stop codon which allows for expression of soluble antibody into bacterial supernatant...
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I am designing an antibody to detect the protein LynA in western blot. Right now I have 8 potential antibodies (SCFVs) that may or may not bind by western blot. I have an apparatus that lets me...
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Hello, I am curious if anybody has insight into doing a helper phage rescue with either hyperphage or M13ko7-d3 (helper phage which are deficient in the pIII protein). [CLARIFICATION: I want to...
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