Farbod Bahreini

4 Questions 5 Answers 0 Followers

Questions related from Farbod Bahreini

Difference between the effect of heated FBS and non heated one?

Hi everyone, I am working with an adhesive fibroblast cell line in roller flasks (Cell Master Bottles). When culturing the cells with FBS that is heated with FBS (around 60 degrees C), the cells...

03 June 2024 7,464 1 View

Does J774A.a cell line change its behavior after a certain time?

Hi everyone, I am measuring the IL-6 secretion in the J774.A.1 cell line upon the activation with a TLR1/2 ligand. In my previous studies, everything seemed normal. But recently I have had some...

19 May 2023 2,416 2 View

Can we reuse T175 flasks for cell culturing?

Dear All, For the environmental purposes and also saving money, can cell culture flasks be reused? I am using an adherent cell line. Whether T75or T175. Thanks!

08 May 2023 5,998 8 View

(In)direct ELISA against murine IL-6?

Dear ResearchGate members, As you know almost all of the commercially produced (available) kits that measure murine IL-6 by ELISA are designed basedon the Sandwich ELISA, that makes the kits very...

05 May 2023 4,308 3 View